The club was founded in 1965.In 2001 they joined the Senior Division of theReading League, going on to win the league in 2006–07.As a result, the club were promoted to Division One East of the Hellenic League. After finishing fourth in theirfirst season, they claimed the runners-up spot in2008–09 season, earning promotion to the Premier Division.The club made its debut in theFA Vasein the 2010–11 season and the2011–12 seasonsaw them play in theFA Cupfor the first time. Their first match was againstWembley, which was the first-ever FA Cup match streamed live onFacebook.The game also led to Ascot's then-record attendance of 1,149.In 2013–14 the club won the Hellenic League Challenge Cup, defeatingArdley United2–1 in the final.
The 2018–19 season saw Ascot win the Challenge Cup for a second time with a 3–1 win against Windsor in the final. In 2022–23 they were Combined Counties Football League Premier Division North champions, earning promotion with 102 points as well as reaching the final of the 2022–23 FA Vase at Wembley Stadium.